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Fig. 7 | Cell & Bioscience

Fig. 7

From: Babao Dan attenuates acute ethanol-induced liver injury via Nrf2 activation and autophagy

Fig. 7

Autophagy activation reduces ethanol-induced liver damage. a Endogenous LC3 expression was detected by western blotting in liver with β-actin as a loading control. The conjugated and unconjugated form of LC3 is referred to as LC3-II and LC3-I, respectively. The protein level of p62 was also analyzed by western blotting. b C57BL/6 mice were pretreated with 3-MA (30 mg/kg) or CQ (50 mg/kg) intraperitoneally 30 min before the treatment of NS or BBD, and ethanol was administrated 1 h later as described in animals and treatments as well as the next steps. Both the latency time to drunkenness (disappear of righting reflex) and the drunken sleep time (polysomnography) were assessed. c Mice were treated as indicated and serum levels of ALT and AST were determined. d Influences of BBD-induced autophagy on serum and hepatic TG level. e One representative liver section performing H&E staining from each group is exhibited. Images are shown in ×200. f Effect of autophagy on liver lipid accumulation in mice administrated with ethanol was demonstrated through representative Oil Red O-stained liver sections (×200 magnification). g ROS level in liver. Results from three replicates were expressed as mean ± SD, n = 10; *P < 0.05, **P < 0.01; ^P < 0.05. NS normal 0.9% saline, EtOH ethanol, BBD 0.5 mg/kg Babao Dan

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