Fig. 6
From: Babao Dan attenuates acute ethanol-induced liver injury via Nrf2 activation and autophagy
BBD-serum induces autophagy in AML-12 cells. a Immunoblots were used to analyze endogenous LC3 expression, and β-actin expression was used as control. Total protein extracts were analyzed by western blotting with antibody against p62. b GFP-tagged LC3 expression vector has been utilized to demonstrate the occurrence of autophagy and was detected using an inverted fluorescence microscope. c Representative electron micrographs (original magnification: ×3000) clearly exhibited the autophagic vacuoles in the cytoplasm content. Experiments were repeated three times. #P < 0.05, ##P < 0.01 compared to control