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Fig. 1 | Cell & Bioscience

Fig. 1

From: Babao Dan attenuates acute ethanol-induced liver injury via Nrf2 activation and autophagy

Fig. 1

BBD protects against ethanol-induced liver injury. The mice were divided randomly into six groups (n = 10/group): control, normal 0.9% saline + ethanol, BBD (0.125 g/kg BW) + ethanol, BBD (0.25 g/kg BW) + ethanol, BBD (0.5 g/kg BW) + ethanol and BBD (0.5 g/kg BW). All mice survived the experimental period until sacrifice. a Both latency time to drunkenness (disappear of righting reflex) and drunken sleep time (polysomnography) were assessed. b Serum enzyme activities of aspartate ALT and AST were measured to reflect acute ethanol-induced liver injure. c Ethanol-induced histopathological changes in livers were presented by representative section photographs (original magnification: ×200) of H&E staining. The hepatic steatosis was also evaluated via hepatic steatosis scores ranging from 0, 1, 2 and 3 under a microscope. Mean rank was obtained for plotting. d Ethanol-induced lipid accumulation was demonstrated through representative Oil Red O-stained sections of livers (×200 magnification). e Liver and serum TG levels were determined as indicated methods. Values presented are from three replicates as mean standard ± deviation (SD), n = 10; ##P < 0.01, ###P < 0.001 compared to control; *P < 0.05, **P < 0.01 compared to NS group; ^P < 0.05. BW body weight, NS normal 0.9% saline, EtOH ethanol, BBD Babao Dan, 0.125 BBD 0.125 mg/kg BBD, 0.25 BBD 0.25 mg/kg BBD, 0.5 BBD 0.5 mg/kg

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