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Fig. 3 | Cell & Bioscience

Fig. 3

From: Urothelial carcinoma associated 1 promotes trophoblast invasion by regulating MMP9

Fig. 3

Knockdown UCA1 inhibits trophoblast outgrowth in extravillous explants by downregulating MMP9 expression. a Placenta explants from HCs (6–10 weeks) were seeded into Matrigel of a 24-well plate. The villi explants were transfected with siCtrl or siUCA1, and a serial of pictures for explants were individually taken using the microscope 24 h and 72 h after transfection. Scale bars: 250 μm. b The outgrowth capacities of the trophoblasts were determined with the ImagePro plus 6.0 software. *P < 0.05; siUCA1 vs siCtrl. ce Placenta explants from HCs were seeded into Matrigel of a 24-well plate. The villi explants were then transfected with control siRNA or siUCA1 and cultured for 48 h. The supernatants were collected for ELISA experiments and gelatin zymography experiments. *P < 0.05; siUCA1 vs siCtrl. f Whole-mount immunofluorescent staining using anti-MMP9 antibody exhibited a notable decrease of the MMP9 level (green) in siUCA1-knockdown group (b) than that of the siCtrl one (a). Scale bars: 25 μm. CK7 level was shown in red color and nuclei were counterstained with DAPI (blue). g Immunohistochemical staining of villous trophoblasts from RM patients (n = 15) and HCs (n = 15) using anti-MMP9 antibody and a HRP IHC kit. Scale bars: 100 μm. h Primary cytotrophoblast cells were freshly collected from the first-trimester villi tissues from either RM patients or HCs, and expression of MMP9 was measured using immunofluorescence staining. Scale bars: 25 μm

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