Fig. 2
From: Optogenetic stimulation inhibits the self-renewal of mouse embryonic stem cells
Characteristics of the ChR2-GFP-V6.5 ESCs. a Immunostaining for the pluripotency markers Oct4 and SSEA1 in ChR2-GFP-V6.5 ESCs. b qRT-PCR analysis of the pluripotency-associated genes Oct4, Sox2, Nanog, Esrrb and Klf4 in ChR2-GFP-V6.5 ESCs and V6.5 ESCs. c AP staining of ChR2-GFP-V6.5 ESCs. d A nude mouse subcutaneously injected with 5*106 ChR2-GFP- V6.5 ESCs on the left side formed a teratoma. (n = 6, *P < 0.05 compared to the wild-type control). e Hematoxylin and eosin staining of teratomas derived from V6.5 ESCs and ChR2-GFP-V6.5 ESCs