Table 1 A comparison of protocols proposed to produce naive hESC
Protocol | Year | Origin | Growth factors | Inhibitors | Transgene expression |
|---|---|---|---|---|---|
Hanna | 2010 | Primed hESC | TGF\(\beta\) - LIF, BMP4 | MEKi, GSKi, JNKi, P38i, PKCi, ROCKi | OCT4, SOX2, KLF4, KLF2 |
Gafni | 2013 | Primed hESC | bFGF, TGF\(\beta\), LIF | MEKi, GSKi, FGFi, JAKi, ALKi, ROCKi | – |
Ware | 2014 | Primed hESC | bFGF | MEKi, GSKi | – |
Theunissen | 2014 | ICM | Activin, LIF | MEKi, GSKi, ROCKi, BRAFi, SRCi | KLF2, NANOG |
Takashima | 2014 | Primed hESC | bFGF, LIF | MEKi, GSKi, PKCi | – |
Valamehr | 2014 | Fibroblast (iPSC) | bFGF | MEKi, GSKi, ROCKi, ALKi | OCT4, SOX2 |
Duggal | 2015 | Primed hESC | bFGF, LIF | MEKi, GSKi, ROCKi | – |
Guo | 2016 | ICM | bFGF, LIF | MEKi, GSKi, ROCKi, PKCi | – |