Fig. 1

CKIP-1 was downregulated in DR tissues and high-glucose treated HRECs. a Real-Time qPCR was used to detect CKIP-1 mRNA levels in DR tissues (N = 20) and normal tissues (N = 10). b Western Blot Was performed to detect CKIP-1 protein levels in DR tissues (The “1#, 2# and 3#” represented 3 individual clinical specimens). c MDA assay kit was used to detect MDA levels, SOD kit was used to detect SOD activity and GSH-Px kit was used to detect GSH-Px activity in DR tissues respectively (n = 3). d The morphology of HRECs treated with low-glucose (5.5 mM) and high-glucose (25 mM) (Scale bar is 200 μm). e Relative CKIP-1 mRNA levels in HRECs treated with low-glucose (5.5 mM) and high-glucose (25 mM) was detected by Real-Time qPCR (n = 3). f Relative CKIP-1 protein levels in HRECs treated with low-glucose (5.5 mM) and high-glucose (25 mM) was detected by Western Blot (The “1#, 2# and 3#” represented 3 individual repetitions). The data above in one experiments were repeated at least 3 times and performed as mean ± standard deviation (SD), *P < 0.05, **P < 0.01 and ***P < 0.001