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Fig. 2 | Cell & Bioscience

Fig. 2

From: Colon cancer cells secrete exosomes to promote self-proliferation by shortening mitosis duration and activation of STAT3 in a hypoxic environment

Fig. 2

Exo-hypoxic conditions promote colon cancer cell line self-proliferation more significantly than Exo-normoxic. a Colon cancer cells SW480 were incubated with different concentrations of Exosomes, including 10 µg/ml, 20 µg/ml and 40 µg/ml, and cell viability was analyzed by MTT assays at 24 h. b Colon cancer cells HCT116 were treated with different concentrations of Exosomes, including 10 µg/ml, 20 µg/ml and 40 µg/ml, and cell viability was analyzed by MTT assays at 24 h. c Western blotting analysis of cyclin D1 expression in SW480 cells treated with the indicated concentrations of SW480‐derived exosomes for 24 h. d Western blotting analysis of cyclin D1 expression in SW480 cells treated with the indicated concentrations of Exo-normoxic and Exo-hypoxic for 24 h. Data are expressed as mean of three replicated experiments. Data are presented as the mean ± standard deviation (*P < 0.05; **P < 0.01; ***P < 0.001)

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