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Fig. 5 | Cell & Bioscience

Fig. 5

From: BH3 mimetic ABT-263 enhances the anticancer effects of apigenin in tumor cells with activating EGFR mutation

Fig. 5

Noxa modulation by apigenin contribute to coadministration-induced cell killing. a H1975 and HCC827 cells were treated with Apg (15 µM), ABT (2 µM) alone or comb for 1 day. Cells were harvested and Bcl-2 family members were detected by Western blotting. b H1975 and HCC827 cells were treated with Apg (15 µM) for up to 12 h, Bcl-2 family members were examined by Western blotting. c H1975 and HCC827 cells infected with the sh-Noxa lentivirus were treated with drugs as described in a. Apoptotic cell death was analyzed (left), and expression of Noxa and cleaved caspase 3 were analyzed by Western blotting (right). d H1975 and HCC827 cells were treated with 10 µM CHX alone or in combination with 15 µM Apg for up to 8 h. Noxa expression was examined by Western blotting (left), and its quantification (right) was examined by ImageJ (the bands densitometry of Noxa were measured by ImageJ and normalized to β-actin, then compared to DMSO-treated control). e H1975 and HCC827 cells were treated with Apg (15 µM) for up to 8 h. The total RNA was extracted and the Noxa mRNA level was quantified by real-time PCR. The data represents mean ± SD (n = 3). **p < 0.01

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