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Fig. 7 | Cell & Bioscience

Fig. 7

From: Regulation of platelet-activating factor-mediated interleukin-6 promoter activation by the 48 kDa but not the 45 kDa isoform of protein tyrosine phosphatase non-receptor type 2

Fig. 7

48 kDa PTPN2 modulates PAF-induced PKD activation in iMoDCs. Immature monocyte-derived dendritic cells (iMo-DCs) were transfected with control siRNAs (siCTRL) or siRNAs against isoforms of PTPN2 (siPTPN2 48 kDa or 45 kDa) on day 4 and 5 before being collected on day 7 for experiments; or not transfected (NT). iMo-DCs were starved for 5 h in RPM1 + 0.2% BSA and stimulated with PAF (1 nM) for indicated times. Whole cell lysates were separated by SDS-PAGE, transferred to nitrocellulose membranes and blotted overnight with anti-pSer910 PKD, anti-PKD and anti-actin antibodies. NT samples have been loaded on the same blots as PTPN2 samples, to serve as controls. a Representative blots and b compilations of at least 3 independent experiments are shown. Significance was established with two-way ANOVA with Sidak post-test. *p < 0.05, **p < 0.01

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Cell & Bioscience

ISSN: 2045-3701

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