Fig. 2

Combination of gemcitabine and VPA regulates Bmi1 expression. PANC-1 and Patu8988 cells were pretreated with 0.5 mM or 5 mM of VPA for 12 h and then cotreated with 5 μm of gemcitabine for 24 h. a The protein level of Bmi1 was measured by western blot analysis. b The nuclear accumulation of Bmi1 was determined by immunofluorescence. The graphs are representative results of three independently repeated experiments