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Fig. 5 | Cell & Bioscience

Fig. 5

From: Label-free quantitative proteomics identifies transforming growth factor β1 (TGF-β1) as an inhibitor of adipogenic transformation in OP9-DL1 cells and primary thymic stromal cells

Fig. 5

TGF-β1 promoted nuclear translocation of β-catenin in OP9-DL1 cells. a Cells were treated with various concentrations of TGF-β1 for 72 h. Immunofluorescence staining was imaged using anti-β-catenin (red), and the nuclei were counterstained with DAPI (blue). Magnification, ×200. b The nuclear/cytoplasmic β-catenin ratio was quantified using ImageJ software and determined by measuring the density of a randomly selected region in the nucleus and cytoplasm. Cells were counted from three different microscopic fields, and results are expressed as mean ± SD. **P < 0.01; *P < 0.05 vs vehicle

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