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Fig. 4 | Cell & Bioscience

Fig. 4

From: Label-free quantitative proteomics identifies transforming growth factor β1 (TGF-β1) as an inhibitor of adipogenic transformation in OP9-DL1 cells and primary thymic stromal cells

Fig. 4

TGF-β1 activated Wnt/β-catenin signaling pathway in OP9-DL1 cells. OP9-DL1 cells were treated with various concentrations of TGF-β1 for 7 days in the presence of rosiglitazone. a LEF1, CCND1, Dvl3, Fzd2 and Axin2 mRNA levels were measured by real-time PCR and normalized to GAPDH. b β-catenin and Axin2 protein levels were assessed by Western blot analysis. The blots shown are representative of three independent experiments. c, d Columns represent mean ± SD from three independent experiments. **P < 0.01; *P < 0.05. Rosi rosiglitazone

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