Fig. 5

IGFBP7 inhibits the phosphorylation and kinase activity of AKT. a Western blotting analysis of phosphorylated AKT (Ser 473 and Thr 308) and total AKT in the indicated cells. α-tubulin was used as a loading control. b The AKT kinase assay measuring AKT activity in vitro was performed. Western blotting analysis of p-GSK-3β (Ser21/9) and total AKT in the indicated cells. All data were obtained from three independent experiments with similar results