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Fig. 6 | Cell & Bioscience

Fig. 6

From: Zika virus antagonizes interferon response in patients and disrupts RIG-I–MAVS interaction through its CARD-TM domains

Fig. 6

ZIKV NS4A blocks VSV-induced IFN production. a 293T cells transfected with IFN-β reporter together with ZIKV-NS4A, DENV-NS4A, ZIKV-prM and IVA-PB1-F2 (500 ng/well) were infected with VSV-GFP for 24 h, and cell lysates were then analyzed for reporter luciferase activity. The data are shown as the mean ± SD derived from three repeat experiments. **p < 0.01 (Student’s t test). b, c 293T cells were transfected with empty vector, and a ZIKV-NS4A-, DENV-NS4A-, ZIKV-prM- or IVA-PB1-F2-expressing plasmid. Twenty-four hours later, the cells were infected with SeV, and the supernatants were UV-treated and used to overlay 293T cells. Twenty-four hours later, the cells were infected with VSV-GFP for 24 h, followed by scoring of the number of GFP-positive cells by flow cytometry (b), and cell images were taken under a fluorescence microscope (c)

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