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Fig. 1 | Cell & Bioscience

Fig. 1

From: Zika virus antagonizes interferon response in patients and disrupts RIG-I–MAVS interaction through its CARD-TM domains

Fig. 1

ZIKV abrogates the activation of type I IFN signaling. a Heatmap of a pre-defined gene list of type I IFN and ISGs that are differentially expressed (DEGs), generated from comparative RNA-seq data among three ZIKV-infected patients (ZIKV patients ac) and three healthy donors. The expression of read-mapped genes normalized across the entire dataset was analyzed based on the RPKM value, and p-values < 0.05 and log2 fold change ≥ 1.5 relative to the control cohort were set as the threshold to assess the statistical significance of the differential gene expression. be Infection with ZIKV reduces type I IFN production after a secondary infection. HFF-1 and SV-HUC-1 cells were infected with mock or ZIKV at an MOI of 1, and 24 h later, respectively, infected with SeV at 100 HAU/ml (b, c) or transfected with 20 μg/ml of poly(I:C) (d, e). IFN-β protein levels were measured using specific ELISA in the supernatants collected at 36 h after secondary-infection from the two cell lines, and cell lysates were harvested for real time RT-PCR to determine the RNA level of SeV (f, g), and for Western blotting analysis to determine the levels of ZIKV envelope protein (E) and GAPDH protein (h, i). Data are shown as the mean ± SD derived from three repeatedexperiments. *p < 0.05, and **indicates p < 0.01 (Student’s t test)

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