Fig. 2

PinX1 and NPM co-localize during S Phase. HeLa cells were synchronized at G1/S boundary by 2 mM hydroxyurea and were fixed at indicated time points. a Flow cytometry analysis of PI stained cells showing cell cycle stages of population. b Immunofluorescence analysis showing localization of PinX1 and NPM at indicated stages of cell cycle after release from hydroxyurea block with anti-NPM (green) and anti-PinX1 (red). DAPI (blue) shows nucleus staining. The white arrows indicate co-localization between PinX1 and NPM