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Fig. 1 | Cell & Bioscience

Fig. 1

From: The PinX1/NPM interaction associates with hTERT in early-S phase and facilitates telomerase activation

Fig. 1

Long-term concurrent knockdown of PinX1 and NPM leads to substantial telomere length decrease in HepG2 cells. PinX1 and/or NPM siRNA were transfected to HepG2 cells every 9 days and the cells were reseeded every 3 days. a Southern blot of transfected HepG2 cells using digosin end-labeled (CCCTAA) oligonucleotide probe. b The standardized mean of the overall telomere length (Kb) for respective cell mass in 1, 5, and 10 PDs measured by Telotool. The dots indicate the average telomere lengths and the bars indicate the range values of telomere restriction fragments. c Western blots showing the expression profile of respective cell mass analyzed by anti-PinX1 and anti-NPM antibodies. GAPDH was used as loading control. d Cell proliferation of the transfected cells was determined with the CCK-8 Kit after cultured for 24-96 h

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