Fig. 5
From: Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection
Schematics of crRNA transfection-mediated gene KO. Our crRNA:tracrRNA transfection method parallels that for siRNA transfection. The RNA components for CRISPR are delivered to Cas9-expressing cells using cationic lipid transfection. They assemble with Cas9 protein to form the active Cas9 RNP which in turn directs target gene cleavage