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Table 1 Primer sequence for sgRNA cloning

From: Functional characterization of SLC26A3 c.392C>G (p.P131R) mutation in intestinal barrier function using CRISPR/CAS9-created cell models

Primer name

Primer sequence, 5′–3′

Human sgRNA1_T

CACCGAATTAACAGTGGGTGAATCG

Human sgRNA1_B

AAACCGATTCACCCACTGTTAATT

Human sgRNA2_T

CACCGCCGATTCTGAGTATGATGGT

Human sgRNA2_B

AAACACCATCATACTCAGAATCGG

Human sgRNA1a_T

CACCGATAAGACCATATAAAATGAC

Human sgRNA1a_B

AAACGTCATTTTATATGGTCTTAT

mouse sgRNA1_t

CACCGAGATAACCAGAGGTAAATGC

mouse sgRNA1_b

AAACGCATTTACCTCTGGTTATCT

mouse sgRNA2_t

CACCGCCAGTTCTGAGTATGATGGT

mouse sgRNA2_b

AAACACCATCATACTCAGAACTGG

Human ssODN

GAAACTGCTCCTGAAACTGCTAGTCCCACCATCATACTCAGAATCCGAAACGGACCTAATTAACAGTGGGTGAATCGTCGTCAGTATATGCCTCTCTAAAGCAC

Human ssODN-a

GAAACTGCTCCTGAAACTGCTAGTCCCACCATCATACTCAGGATAGGCAACGGACCTAATTAACAGTGGGTGAATCGTCGTCAGTATATGCCTCTCTAAAGCACATTGTCTTTCAACCACAGAATAAGACCATATAAAATGACTCGCAAGGCTGGGCGTGGTGGCTCACG

mouse ssODN

GTAACGACAACTCCCACCATCATACTCAGAACTCGAAATGGACCTAGATAACCAGAGGTAAATGCTCGTCAGTAGGTGCCTCCCTACGCCCG