Fig. 6

MiR-150-5p reverses the effects of MALAT1 or AKT3 on proliferation and apoptosis in OA chondrocytes. a Western blot was performed to detect the protein level of AKT3 in chondrocytes transfected with si-AKT3, AKT3 overexpression plasmid, or their negative controls before IL-1β treatment. b, c MTT assay and flow cytometry were performed to detect cell proliferation ability and apoptosis, respectively. Chondrocytes were transfection with NC + miR-NC, MALAT1 + miR-NC, NC + miR-150-5p, MALAT1 + miR-150-5p, miR-NC + AKT3, miR-150-5p + AKT3, anti-NC + anti-NC, si-MALAT1 + anti-NC, si-NC + anti-miR-150-5p, si-MALAT1 + anti-miR-150-5p, anti-NC + si-AKT3, and miR-150-5p + si-AKT3 before IL-1β treatment. *P < 0.05