Fig. 2

Knockdown of MALAT1 inhibited the proliferation and promoted cell apoptosis of hepatocellular carcinoma cells via miR-142-3p. The expression of MALAT1 in HepG2 and SMMC-7721 cells was knocked down by transfecting with MALAT1 siRNAs or si-control. NC was used as the control. The miR-142-3p inhibitor was used to knock down the level of miR-142-3p. a Q-PCR was used to analyze the level of miR-142-3p in HepG2 cells after transfection with MALAT1 siRNAs or control siRNAs (NC). b, c Cell viability was assessed by CCK-8 assay after the indicated transfection in HepG2 and SMMC-7721 cells. d The cell proliferation of HepG2 and SMMC-7721 cells after the indicated transfection was assessed by EdU assay. Representative images of the cells are shown. Scale bar, 100 μm. e, f Cell apoptosis of HepG2 and SMMC-7721 cells after the indicated transfection was evaluated by flow cytometry. Data represent three independent experiments (mean and SEM of triplicate samples). *P < 0.05, **P < 0.01. ***P < 0.001. SEM, standard error of the mean