Fig. 6
From: Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesis
The subnuclear localization of H3K27me3 alters from the nuclear periphery to interior nuclear after Emerin is knockdown in C2C12 myoblasts. a IF assays using C2C12 myoblasts with exogenous Flag-Msx1. Emerin was knockdown in C2C12 myoblasts using epiCRISPR/Cas9 system. H3K27me3 was no longer localized at the nuclear periphery after Emerin was knockdown in C2C12 myoblasts. b IF using 293T cells with exogenous Flag-Msx1. H3K27me3 distributed at the interior nuclear in 293T cells. c Quantification of H3K27me3 localization in C2C12 myoblasts was done using ImageJ. d Quantification of H3K27me3 localization in 293T cells was done using ImageJ. Scale bars represented 10 µm