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Fig. 6 | Cell & Bioscience

Fig. 6

From: Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesis

Fig. 6

The subnuclear localization of H3K27me3 alters from the nuclear periphery to interior nuclear after Emerin is knockdown in C2C12 myoblasts. a IF assays using C2C12 myoblasts with exogenous Flag-Msx1. Emerin was knockdown in C2C12 myoblasts using epiCRISPR/Cas9 system. H3K27me3 was no longer localized at the nuclear periphery after Emerin was knockdown in C2C12 myoblasts. b IF using 293T cells with exogenous Flag-Msx1. H3K27me3 distributed at the interior nuclear in 293T cells. c Quantification of H3K27me3 localization in C2C12 myoblasts was done using ImageJ. d Quantification of H3K27me3 localization in 293T cells was done using ImageJ. Scale bars represented 10 µm

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