Fig. 4

Emerin contributes to the nuclear periphery localization of Msx1. a–d Knockdown Emerin in C2C12 myoblasts using siRNAs. a The expression levels of Emerin after using different siRNAs to knockdown Emerin in C2C12 myoblasts. b mRNA levels of Emerin after using different siRNAs to knockdown Emerin in C2C12 myoblasts. Data are shown as the relative expression level in cells with the Emerin siRNA or the control. Values are the mean ± SD. ***p < 0.0001, **p < 0.001, *p < 0.01. c The expression levels of Emerin after using Emerin#2 siRNA in different transfection concentrations to knockdown Emerin in C2C12 myoblasts. d mRNA expression levels of Emerin after using Emerin#2 siRNA in different transfection concentrations to knockdown Emerin in C2C12 myoblasts. Data are shown as the relative expression level in cells with the Emerin siRNA or the control. Values are the mean ± SD. ***p < 0.0001, **p < 0.001, *p < 0.01. e, f Knockdown Emerin in C2C12 myoblasts using epiCRISPR/Cas9 system. e The expression levels of Emerin using epiCRISPR/Cas9 system to knockdown Emerin in C2C12 myoblasts. f Schematic diagram of two guide RNAs. g–j IF data showing the subnuclear localization of Msx1 altered from the nuclear periphery to interior nuclear after Emerin was knockdown in C2C12 myoblasts. g IF assays performed in C2C12 myoblasts transfected with Emerin#2 siRNA. h IF assays performed in C2C12 myoblasts transfected with epiCRISPR/Cas9 plasmids. i–j Quantification of Msx1 localization from G and H was done using ImageJ. Scale bars represented 10 µm