Fig. 2

Msx1 associates with Emerin via the homeodomain, regardless of DNA binding ability. a C2C12 myoblasts were overexpressed with Flag-Msx1 or Myc-Msx1. Whole cell lysates of C2C12 myoblasts with overexpressed Flag-Msx1 or Myc-Msx1 were immunoprecipitated with anti-Flag beads or anti-c-Myc beads respectively followed by immunoblotting for Emerin or other proteins as indicated. H3K9me2 was a positive control. b 239T cells overexpressed with exogenous Flag-Msx1 were used for Co-IP. Whole cell lysates of 293T cells with overexpressed Flag-Msx1 were immunoprecipitated with anti-Flag beads followed by immunoblotting for Emerin, Flag and β-Actin. c Lysates of embryonic forelimb (E11.5) were immunoprecipitated with anti-Msx1 antibody followed by immunoblotting for Emerin and Msx1. d Domain mapping analyses. 293T cells overexpressed with Msx1 protein fragments were used for immunoprecipitation followed by immunoblotting for Emerin, Flag and β-Actin. e Schematic representation of Msx1 protein fragments according to its functional domain. f 293T cells were overexpressed with exogenous Msx1 or Msx1-A (K174A, R176A and F179A). Whole cell lysates were immunoprecipitated with Anti-Msx1 antibody followed by immunoblotting for Emerin, Msx1 and β-Actin