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Fig. 2 | Cell & Bioscience

Fig. 2

From: The Cdc42 effectors Gic1 and Gic2 regulate polarized post-Golgi secretion

Fig. 2

Localization of the exocyst components in the sec3∆N gic1∆ gic2∆ triple mutant. a Polarity of exocyst components are disrupted in sec3∆N gic1∆ gic2∆ triple mutant, but not in sec3∆N single mutant and gic1∆ gic2∆ double mutant. The exocyst components in wild type, sec3∆N, gic1∆gic2∆, and sec3∆N gic1∆ gic2∆ mutant cells were GFP tagged by chromosomal integration. Cells were grown to log phase at 25 °C and then fixed for fluorescence microscopy. Scale bar, 5 μm. GFP-tagged Sec5, 8, and 70 and Exo84 remained polarized to the bud tip in wild type, sec3∆N, gic1∆gic2∆, but were no longer polarized in sec3∆N gic1∆ gic2∆ triple mutants. b Quantification of the percentage of cells with polarized exocyst subunits in (a). 100 cells were counted per group. The representative results from three independent experiments are shown

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