Fig. 1
From: The Cdc42 effectors Gic1 and Gic2 regulate polarized post-Golgi secretion
The exocyst complex co-immunoprecipitates with Gic2. Yeast strains expressing Sec8-myc or Exo84-myc were transformed with GFP-tagged Gic2. Cells were immunoprecipitated with polyclonal anti-GFP antibody, and probed with anti-myc monoclonal antibody or anti-GFP monoclonal antibody in western blot. Top panel shows the full-length Sec8-myc and Exo84-myc in the lysates. The middle panel shows that Sec8-myc and Exo84-myc were co-immunoprecipitated in cells expressing GFP-tagged Gic2, but not in cells expressing un-tagged Gic2. The bottom panel shows the Gic2-GFP immunoprecipitated by the anti-GFP polyclonal antibody. GFP-tagged Gic2 and cells expressing un-tagged Sec8 or Exo84 were used as negative control. The representative results from three independent experiments are shown