Table 1 Primer sequences used for real-time PCR
Gene | Sequences (5′-3′) | Annealing Temp (oC) | Amplicon length (bp) |
|---|---|---|---|
Vimentin | F: CAGATTGGCTGAAATGGATGAGAA R: TAGGTGGCGATGTCAATGTCAA | 62 | 173 |
ZEB1 | F: CTGGAGAAAAGCCCTATCAATGT R: CTGTCAACATCCTGGTCCCTTGT | 62 | 244 |
Snail | F: GAGTTTAAATTCCAGCTGCC R: CAGAGTCCCAGATGAGCATT | 62 | 109 |
VE-cadherin | F: TGAAGAATTACGAGTCGGAC R: CACAGTCGGATGTGTCGGTGC | 65 | 129 |
18S RNA | F: TCGGCTGGAGAAGAGCTACG R- GTACTTTCGTGGATGTTACA | 57 | 131 |