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Fig. 1 | Cell & Bioscience

Fig. 1

From: Harman and norharman, metabolites of the entomopathogenic fungus Conidiobolus coronatus (Entomophthorales), affect the serotonin levels and phagocytic activity of hemocytes, insect immunocompetent cells, in Galleria mellonella (Lepidoptera)

Fig. 1

Serotonin in hemolymph collected from G. mellonella larvae 1 h after topical application of norharman and harman. a Immunofluorescence localization of serotonin in hemocytes. β-Actin (green) was stained by ActinGreen 488 ReadyProbes Reagent. Cell nuclei (blue) were stained with Hoechst. Serotonin (red) was detected using Mouse Serotonin Monoclonal Antibody (5HT-H209) and Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594. b Serotonin concentration in hemolymph determined by ELISA kit. Test was performed in three independent replicates; n = 20. 1N—norharman 750 ppm, 2N—norharman 1000 ppm, 3N—norharman 1250 ppm, 1H—harman 750 ppm, 2H—harman 1000 ppm, 3H—harman 1250 ppm; ***p ≤ 0.001, **p ≤ 0.01; scale bar 25 µm

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Cell & Bioscience

ISSN: 2045-3701

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