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Table 1 Relative concentration of Smad2/3 expression in western blot test

From: MFAP5 promotes basal-like breast cancer progression by activating the EMT program

 

Sample

NC

MEAP5

NC + SB431542

MFAP5 + SB431542

NC + ly411575

MFAP5 + ly411575

p-Smad2/Smad3

Gray value

0

302,830

0

126,610

0

206,209

0

246,130

0

76,568

0

171,906

0

292,443

0

79,480.3

0

186,458

Average

0

280,468

0

94,219

0

188,191

Gray value of each molecule relative to beta-actin (G1)

0.00

0.91

0.00

0.28

0.00

0.63

Compared with control group

0.00

0.10

0.00

0.08

0.00

0.06

Compared with control group (G1/NC)

0.00

9.29

0.00

2.89

0.00

6.51

Internal reference

Gray value

365,609

302,497

300,448

314,709

340,436

326,191

303,144

316,665

357,508

336,392

292,601

284,270

335,737

309,839

352,063

352,295

287,281

279,450

Average

334,830

309,667

336,673

334,465

306,773

296,637

Compared with control group

0.09

0.02

0.09

0.06

0.10

0.09