Fig. 7
From: MFAP5 promotes basal-like breast cancer progression by activating the EMT program
MPAP5 regulated epithelial–mesenchymal transition of BLBC cells through TGF-β and Notch pathways. BT20-LV-vehicle and BT20-LV-MFAP5 cells were treated with SB431542 or ly-411575 as indicated for 24 h and then collected to test the protein levels of E-cadherin, N-cadherin and Type I Collagen by Western blotting and the mRNA levels of Slug, Snail, Zeb1, Zeb2 and Twist by RT-PCR. a Overexpression of MFAP5 reduced the protein level of E-cadherin and increased N-cadherin and Collagen I expression in BT20 cells. SB431542 and ly-411575 partly abolished the inhibitory effect of MFAP5 on E-cadherin and the promoting role on N-cadherin and collagen I. **P < 0.01 vs LV-vehicle control; ##P < 0.01 vs LV-MFAP5 control. b Compared with LV-vehicle group, the mRNA levels of Slug, Snail, Zeb1, Zeb2 and Twist were significantly increased in BT20 cells of LV-MFAP5 group which were decreased by SB431542 and ly-411575. **P < 0.01 vs LV-vehicle control; ##P < 0.01 vs LV-MFAP5 control