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Fig. 3 | Cell & Bioscience

Fig. 3

From: Hypoxia destroys the microstructure of microtubules and causes dysfunction of endothelial cells via the PI3K/Stathmin1 pathway

Fig. 3

Stathmin1 is down-regulated after hypoxia and H2O2 treatment. HUVECs were cultured under normoxia, hypoxia or H2O2 conditions with or without pretreatment of 1 mM NAC or 1 μM wortmannin for 30 min. a Total cell lysates were immunoblotted with antibodies to acetyl α-tubulinK40, stathmin1 and P85. b The mRNA level of stathmin1 was detected with RT-qPCR. c Western blot of acetyl α-tubulinK40, PI3K and P85 (loading control) of HUVEC cells treated with PI3K siRNAs. d HUVEC cells were treated with hypoxia for 8 h with or without protein synthesis inhibitor cycloheximide and the transcription inhibitor actinomycin D, total cell lysates were immunoblotted with antibodies to acetyl α-tubulinK40 and P85. Representative results from three independent experiments were depicted here. The relative intensity of band was normalized to P85. The data are presented as the mean ± SEM. *P < 0.05

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