Fig. 3

Stathmin1 is down-regulated after hypoxia and H₂O₂ treatment. HUVECs were cultured under normoxia, hypoxia or H₂O₂ conditions with or without pretreatment of 1 mM NAC or 1 μM wortmannin for 30 min. a Total cell lysates were immunoblotted with antibodies to acetyl α-tubulin^K40, stathmin1 and P85. b The mRNA level of stathmin1 was detected with RT-qPCR. c Western blot of acetyl α-tubulin^K40, PI3K and P85 (loading control) of HUVEC cells treated with PI3K siRNAs. d HUVEC cells were treated with hypoxia for 8 h with or without protein synthesis inhibitor cycloheximide and the transcription inhibitor actinomycin D, total cell lysates were immunoblotted with antibodies to acetyl α-tubulin^K40 and P85. Representative results from three independent experiments were depicted here. The relative intensity of band was normalized to P85. The data are presented as the mean ± SEM. *P < 0.05