Fig. 4

Osteogenic differentiation inhibits cellular proliferation. a Cell proliferation was assessed using an EdU labeling assay before and after osteogenic differentiation in the AM-MSCs, UC-MSCs, CM-MSCs, and DC-MSCs. The images display EdU staining (red color) merged with Hoechst 33342 staining (blue color). Scale bar = 100 μm. b The graph represents the percentage of EdU-positive cells. Error bars represent the SD, ***p ≤ 0.001 for differences between the two experimental groups were applied using an unpaired two-tailed Student’s t-test