Fig. 3

qRT-PCR was performed to analyze the potency and kinetics of osteogenic differentiation in a the AM-MSCs, b CM -MSCs, c UC -MSCs, and d DC-MSCs at 0, 7, 14, and 21 days. The y-axis represents the relative mRNA fold change, which was calculated using the 2^−ΔΔCt formula with β-actin as internal control. Error bars represent the SD. The colored bar indicates the differentiation processes of PD-MSCs into osteogenic lineages. The yellow bar represents the undifferentiation statue, the red bar represents the osteoprogenitor statue (BMP-6 and Runx2), the purple bar represents the osteoblast statue (COL1A1 and osteocalcin), and the blue bar represents the osteocyte statue (FGF23 and Sclerostin)