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Fig. 3 | Cell & Bioscience

Fig. 3

From: PNS-R1 inhibits Dex-induced bronchial epithelial cells apoptosis in asthma through mitochondrial apoptotic pathway

Fig. 3

PNS-R1 attenuates Dex-induced apoptosis of 16HBE and inhibits the activation of mitochondrial apoptotic pathway. a The TUNEL assay was used to measure apoptosis of 16HBE. b Statistical results of TUNEL positive cells percentage. Percentages of apoptosis (TUNEL-positive) cells were evaluated by using ImageJ software. Total number of cells was detected by DAPI. c The Flow cytometry of Annexin V-PI apoptosis-detection kit revealed that apoptosis cells ratio of 16HBE. d Statistical results of cell apoptosis rate detected by flow cytometry. e Western blot was used to detect expressions of Bcl-2, Bax, Cyt-c, Apaf-1, cleaved-caspase3 and cleaved-caspase9 proteins in 16HBE cells. f The results of the western blot were quantified. Data represent the average ± SD. Significant differences between two are indicated as *P < 0.01, Dex group vs Dex + PNS-R1 group

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