Fig. 2From: PNS-R1 inhibits Dex-induced bronchial epithelial cells apoptosis in asthma through mitochondrial apoptotic pathwayPNS-R1 decreases apoptosis of bronchial epithelial cells induced by Dex and inhibits the activation of mitochondrial apoptotic pathway in asthmatic mice. a The TUNEL assay revealed apoptosis of bronchial epithelial cells in different mice groups. b Statistical results of TUNEL positive cells percentage. Percentages of apoptosis (TUNEL-positive) cells were evaluated by using ImageJ software. Total number of cells was detected by DAPI. c Western blot was used for detection of mitochondrial apoptotic pathway including Bcl-2, Bax, Cyt-c, Apaf-1, cleaved-caspase3 and cleaved-caspase9 proteins. d The results of the western blot were quantified. Data represent the average ± SD. Significant differences between two are indicated as *P < 0.01, Dex group vs Dex + PNS-R1 groupBack to article page