Fig. 4

Effect of NVD treatment of HCT116 and HT29 (a, b) cells on protein expression of cdc2, WAF1/p21, cdk 2, 4 cyclin A, B1, E2 and (c, d) showing active Caspase 3, 7, 9 and PARP. Cells were administrated with NVD (20, 40 and 60 μM) for 24 h and 48 h and harvested. Total cell lysates were prepared and 40 μM proteins was subjected to SDS page followed by immunoblot analysis and chemiluminescence detection. Equal loading of protein was verified by stripping the Immunoblot and again probing it for Actin. The immunoblots shown here are representative of three individual experiments with similar results