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Fig. 4 | Cell & Bioscience

Fig. 4

From: Scutellarin protects human retinal pigment epithelial cells against hydrogen peroxide (H2O2)-induced oxidative damage

Fig. 4

Scutellarin elevated the activation of JAK2/STAT3 signaling pathway in H2O2-induced ARPE-19 cells. a Effect of scutellarin on JAK2/STAT3 signaling pathway in H2O2-induced ARPE-19 cells. Cells were challenged with H2O2 (1 mM) for 24 h in the presence or absence of scutellarin (100 μM). Expressions of p-JAK2, JAK2, p-STAT3, and STAT3 were measured using western blot. b Quantification analysis of p-JAK2/JAK2 and p-STAT3/STAT3. Cells were pretreated with scutellarin (100 μM) and/or AG90 (40 μM) or si-JAK2 for 2 h, and then challenged with H2O2 (1 mM) for 24 h. c, d Effects of AG90 on cell viability and apoptosis. e Effect of AG90 on ROS level. *p < 0.05 versus the control cells; #p < 0.05 versus ARPE-19 cells exposed to H2O2; &p < 0.05 versus H2O2 + scutellarin (100 μM) group; ap < 0.05 versus H2O2 + scutellarin (100 μM) group

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