Fig. 4
From: Critical role of Tim-3 mediated autophagy in chronic stress induced immunosuppression
Tim-3 mediates immune suppression following chronic stress through modulating autophagy. The primary splenocytes we isolated from stressed or unstressed mice were transfected with Tim-3-Si or NC-Si and cell supernatants were obtained. The levels of IFN-γ (a), IL-17 (b), IL-10 (c), and IL-4 (d) cytokines were detected by ELISA. N = 5 per group. *P < 0.05, **P < 0.01 compared with indicated groups. e Total splenocytes were enumerated with a hemocytometer