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Fig. 3 | Cell & Bioscience

Fig. 3

From: Critical role of Tim-3 mediated autophagy in chronic stress induced immunosuppression

Fig. 3

Stress promotes expression of Tim-3 and autophagy-related proteins in CD4+ T cell. We obtained CD4+ T cells from splenocytes of mice that were subjected to chronic stress for 12 h or unstressed control mice. a The purity of resulting recovered CD4+ T cells was testified under a fluorescence microscope by immunofluorescence and b total splenic CD4+ T cells from stressed and unstressed mice were enumerated. c Apoptosis of CD4+ T cells was examined by Annexin V-FITC and PI staining. d Cellular lysates were extracted from the isolated CD4+ T cells. The Tim-3 expression and autophagy level were examined by Western blot analysis. N = 3 per group. *P < 0.05, **P < 0.01 compared with indicated groups

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