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Fig. 4 | Cell & Bioscience

Fig. 4

From: Long non-coding RNA BLACAT1 promotes breast cancer cell proliferation and metastasis by miR-150-5p/CCR2

Fig. 4

miR-150-5p suppressed CCR2 expression in breast cancer cells. a The predicted target genes of miR-150-5p. b The mRNA levels in SKBR3 cells with miR-150-5p transfection. SKBR3 cells were transfected with miR-150-5p and total RNA was extracted for qRT-PCR analysis. c MiR-150-5p significantly inhibited luciferase activity of wild type reporter for CCR2, The putative sequences of miR-150-5p and CCR2 with a binding site. d MiR-150-5p down-regulated CCR2 mRNA levels in SKBR3 and MDA-MB-231 cells. SKBR3 and MDA-MB-231 cells were transfected with miR-150-5p mimics and total RNA was extracted for real time RT-PCR analysis. e MiR-150-5p down-regulated CCR2 protein levels in SKBR3 and MDA-MB-231 cells by western blotting. SKBR3 and MDA-MB-231 cells were transfected with miR-150-5p mimics and total protein was extracted for western blot analysis

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