Table 1 Primer sequence used in qPCR assays
From: Transmembrane protein 108 involves in adult neurogenesis in the hippocampal dentate gyrus
Gene name | Forward primer sequence (5′–> 3′) | Reverse primer sequence (5′–> 3′) |
|---|---|---|
Tmem108 | CCTGAGCTACTGGAACAATGCC | CAGTGTCTCGATAGTCGCCATTG |
Gapdh | CATCACTGCCACCCAGAAGACTG | ATGCCAGTGAGCTTCCCGTTCAG |
β-Actin | CATTGCTGACAGGATGCAGAAGG | TGCTGGAAGGTGGACAGTGAGG |
Disc1 | TGGTCGAGGATGGCGATTACGA | AGAGCAGGTTGCTGTGAAGGCA |
β-Catenin | GTTCGCCTTCATTATGGACTGCC | ATAGCACCCTGTTCCCGCAAAG |
Apc | GTGGACTGTGAGATGTATGGGC | CACAAGTGCTCTCATGCAGCCT |
Gsk3β | GAGCCACTGATTACACGTCCAG | CCAACTGATCCACACCACTGTC |
PI3K | CAAACCACCCAAGCCCACTACT | CCATCAGCAGTGTCTCGGAGTT |
Axin1 | GTCCAGTGATGCTGACACGCTA | GCCCATTGACTTGGATACTCTCC |
Axin2 | ATGGAGTCCCTCCTTACCGCAT | GTTCCACAGGCGTCATCTCCTT |