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Fig. 3 | Cell & Bioscience

Fig. 3

From: IL-33 ameliorates experimental colitis involving regulation of autophagy of macrophages in mice

Fig. 3

Treatment with IL-33 ameliorates experimental colitis through enhancing autophagy in mice. BALB/c mice were given 2.5 mg/mouse TNBS (dissolved in 50% ethanol), TNBS + PBS, TNBS + IL-33, or the vehicle ethanol intrarectally, respectively. All the mice were sacrificed on fourth day. a Body weight changes were monitored between zeroth to fourth day in different groups of mice. b Colon lengths were measured. c Histological analysis (hematoxylin and eosin staining) of mice colons was determined under microscope (original magnification, ×200). d Extracts from colons were subjected to LC3 western blotting to evaluate for treatment with TNBS, TNBS + PBS, TNBS + IL-33 versus the vehicle ethanol. Values were mean ± SD. The asterisk indicates p < 0.05. Data were presented as means + SD of three to six individual mice per group and represent > three independent experimental repeats

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