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Fig. 1 | Cell & Bioscience

Fig. 1

From: Dynamics expression of DmFKBP12/Calstabin during embryonic early development of Drosophila melanogaster

Fig. 1

The expression patterns of DmFKBP12 protein and its mRNA during Drosophila embryogenesis within four stages syncytial blastoderm, cellular blastoderm, early and late gastrulation. a Expression of DmFKBP12 protein by Western blot at the four stages during Drosophila embryonic development. b Displays RT-PCR analysis of the expression of DmFKBP12 mRNA during different embryonic developmental stages. (1) Syncytial blastoderm; (2) Cellular blastoderm; (3) Early gastrulation stage; (4) Late gastrulation stage; (5) Positive control; (6) Negative control. β-Tubulin served as internal control. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001. The two fragments of DmRyR domain were sub-cloned into pGME-T easy examined as c. The digestion pattern of restriction enzymes exhibited of clones with the cDNA DmRyR domain (I + II) (c-4, -5 and -6) compared to blank vector (c-1, -2 and -3). For the cDNA DmRyR domain (III + IV), the digestion pattern of clones showed as the plasmids with cDNA DmRyR domain (III + IV) (c-9, -11 and -12) compared to blank vector (c-7, -8 and -10)

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