Fig. 4

Role of TRAF2 in regulating type I IFN Induction. qRT-PCR analysis of Ifna and Ifnb expression in WT or TRAF2^–/– primary MEFs infected with Sev (a) or HSV-1 (b). c, d qRT-PCR analysis of Ifna and Ifnb expression in BMDMs derived from WT or Traf2^MKO mice stimulated with LPS (c) or poly(I:C) (d). e Immunoblot analysis of the indicated proteins in whole-cell lysates of WT or TRAF2^MKO BMDMs stimulated with LPS for the indicated time points. f, g qRT-PCR analysis of Ifna and Ifnb mRNA expression in WT and Traf2^MKO BMDM cells infected with Sev (f) or HSV-1 (g). Data are representative of three independent experiments, and statistical analyses are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. ns nonsignificant