Fig. 3

Stimulus-specific function of TRAF3 in regulating type I IFN induction in BMDCs. a, b qRT-PCR analysis of Ifna and Ifnb expression in BMDCs derived from WT or Traf3^DC-KO mice stimulated with LPS (a) or poly(I:C) (b). c Immunoblot analysis of the indicated proteins in whole-cell lysates of WT or Traf3^DC-KO BMDCs stimulated with LPS for the indicated time points. qRT-PCR analysis of Ifna and Ifnb mRNA expression in WT and Traf3^DC-KO BMDCs infected with VSV (d), Sev (e) or HSV-1 (f) or treated with lipofectamine-transfected poly(I:C) (g) or cGAMP (h) for the indicated time periods. Data are representative of three independent experiments, and statistical analyses are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. ns nonsignificant