Fig. 2

Cell type-specific function of TRAF3 in regulating type I IFN induction. qRT-PCR analysis of Ifna and Ifnb mRNA expression in WT and Traf3^–/– primary MEFs infected with VSV (a) or SeV (b) or treated with transfected with poly(I:C) (c) for the indicated times. d Immunoblot analysis of the indicated phosphorylated (P−) and total proteins in the cytoplasmic (CE) and nuclear (NE) extracts of WT and Traf3^–/– primary MEFs infected with VSV (M.O.I = 10) for the indicated time period. qRT-PCR analysis of relative Ifna and Ifnb mRNA amounts in WT and Traf3^–/– MEF cells infected with HSV-1 (e) or treated with lipofectamine-transfected cGAMP (f), lipofectamine-transfected ISD (g), or stimulated with DMXAA (h) for the indicated time periods. i, j Immunoblot analysis of the indicated proteins in whole-cell lysates of WT or Traf3^–/– MEFs infected with HSV-1 (i) or lipofectamine-transfeted cGAMP (j) for the indicated time periods. qRT-PCR analysis of Ifna and Ifnb mRNA relative level in BMDMs derived from WT or Traf3^MKO mice infected with VSV (k), SeV (l), HSV-1 (m) or stimulated with LPS (n) or poly(I:C) (o). p Immunoblot analysis of the indicated proteins in whole-cell lysates of WT or Traf3^MKO BMDMs stimulated with LPS for the indicated time periods. All qRT-PCR data are presented as fold relative to the amount of an internal control, Actb. Data are representative of three to four independent experiments, and statistical analyses are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. ns nonsignificant