Fig. 5

PLAG inhibits gemcitabine-induced phosphorylation of PLCβ3, PKCα/βII and PKCδ, which are upstream signaling regulators of p47phox phosphorylation. a THP-1 cells were treated with 10 μg/ml of gemcitabine for 0 to 2 h, and the phosphorylation of PLCβ3, PKCα/βII, PKCδ and p47phox was analyzed by western blot. b The effect of PLAG on gemcitabine-induced phosphorylation of PLCβ3, PKCα/βII and PKCδ was analyzed by western blot. THP-1 cells were treated with c PLC inhibitor (U73122) and d PKC inhibitor (Rottlerin) 1 h before gemcitabine treatment, and the mRNA and the protein level of CXCL8 and e p47phox and p38 MAPK phosphorylation were determined. *p < 0.05, **p < 0.01, ***p < 0.001