Fig. 3
PLAG decreases gemcitabine-induced ROS production that mediates neutrophil migration. a NAC (50 mg/kg) was administered intraperitoneally at a dose of 50 mg/kg 1 h before gemcitabine administration. Neutrophils in the blood and the peritoneal cavity were counted using CBC analysis. Each group contains five mice, and the bars represent the mean ± SD. b Transmigration of dHL-60 cells towards THP-1 conditioned medium was decreased by NAC treatment. c The effect of NAC on gemcitabine-induced CXCL8 mRNA expression was analyzed by RT-PCR. d BMDMs and THP-1 cells were treated with various doses of NAC 1 h before gemcitabine treatment, and the protein level of MIP-2 or CXCL8 in the supernatants was determined by ELISA. e, f The level of intracellular ROS in BMDMs and THP-1 cells were analyzed by flow cytometry. g CM-H2DCFDA fluorescence imaging of ROS in BMDMs and THP-1 cells using a confocal microscope. ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001