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Fig. 2 | Cell & Bioscience

Fig. 2

From: 1-Palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG) attenuates gemcitabine-induced neutrophil extravasation

Fig. 2

PLAG inhibits gemcitabine-induced MIP-2(CXCL8)-CXCR2-mediated neutrophil chemotaxis. a Reparixin was diluted in mineral oil and administered intraperitoneally at a dose of 50 mg/kg 1 h before gemcitabine administration. Neutrophils in the blood or in the peritoneal cavity were counted using CBC analysis. Each group contains five mice, and bars represent the mean ± SD. b Peritoneal fluids were harvested, and the protein level of MIP-2 was evaluated by ELISA. BMDMs and THP-1 cells were treated with various doses of PLAG and stimulated with gemcitabine (10 μg/ml). c The mRNA level of MIP-2 or CXCL8 was analyzed by RT-PCR, and d the protein level of MIP-2 or CXCL8 in the culture medium was determined by ELISA. e The effect of PLAG on gemcitabine-induced phosphorylation of ERK, p38 MAPK and JNK was analyzed by western blot in THP-1 cells. The transmigration of differentiated HL-60 cells towards THP-1 conditioned medium was decreased by f PLAG and g reparixin treatment. ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001

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